Figure 5

Representative photomicrographs showing nodose neurons retrogradely labeled from the trachea with fluorogold (FG) overlaid with immunoreactivity for (A) α1 Na+/K+ ATPase, (B) α3 Na+/K+ ATPase, (C) vGlut1, (D) vGlut2, (E) NKCC1 or (F) negative control (neg). In panels A and B, the arrows point to FG traced neurons that are immunoreactive for α1 or α3 Na+/K+ ATPase, the arrow heads show traced neurons that are not immunoreactive for α1 or α3 Na+/K+ ATPase and the asterisks show FG-negative neurons that are immunolabeled for α1 or α3 Na+/K+ ATPase. Traced neurons appear red in panel A as the tissue underwent secondary immunoprocessing for FG. Scale bar represents 40 μm.