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Table 1 Details of the primary and secondary antibodies used for immunohistochemical staining.

From: Immunohistochemical characterization of nodose cough receptor neurons projecting to the trachea of guinea pigs

  Host Dilution Source
Primary Antibodies – Transporters    
   α1 Na+/K+ ATPase (clone 05–369) Mouse 1:100 Millipore, Australia.
   α3 Na+/K+ ATPase (clone XVIF9-G10) Mouse 1:400 Biomol, PA, USA.
   NKCC1 Rabbit 1:1000 Gift Dr RJ Turner, National Institute of Dental and Craniofacial Research, USA.
   vGLUT1 (catalogue# 135 302) Rabbit 1:2000 Synaptic Systems Goettingen, Germany.
   vGLUT2 (catalogue# 135 402) Rabbit 1:2000 Synaptic Systems Goettingen, Germany.
Primary Antibodies – Neurotransmitters    
   CGRP (catalogue# RPN 1842) Rabbit 1:4000 Amersham, UK.
   Neuronal nitric oxide synthase (nNOS) Sheep 1:4000 Gift Dr Colin Anderson, University of Melbourne, Australia.
   Somatostatin (catalogue# AB5494) Rabbit 1:100 Millipore, Australia.
   Substance P (clone NC1) Rat 1:200 Millipore, Australia.
Primary Antibodies – Cytosolic Proteins    
   Calbindin D28k (number CB-38A) Rabbit 1:1000 Swant Bellinzona, Switzerland.
   Calretinin (number 7699/4) Rabbit 1:1000 Swant Bellinzona, Switzerland.
   Neurofilament 160KD (clone NN18) Mouse 1:400 Millipore, Australia.
   Parvalbumin (number 235) Mouse 1:400 Swant Bellinzona, Switzerland.
Secondary Antibodies (IgG, H+L, 2 mg/ml)    
   AlexaFluor 488 or 594 anti-goat Donkey 1:200 Molecular Probes Eugene, OR, USA
   AlexaFluor 488 or 594 anti-mouse Donkey 1:200 Molecular Probes Eugene, OR, US
   AlexaFluor 488 or 594 anti-rabbit Donkey 1:200 Molecular Probes Eugene, OR, USA
   AlexaFluor 488 anti-rat Goat 1:200 Molecular Probes Eugene, OR, USA
  1. Note, AlexaFluor 488 and 594 are green and red fluorphores, respectively.