Table 1 Details of the primary and secondary antibodies used for immunohistochemical staining.
| Â | Host | Dilution | Source |
|---|---|---|---|
Primary Antibodies – Transporters |  |  |  |
   α1 Na+/K+ ATPase (clone 05–369) | Mouse | 1:100 | Millipore, Australia. |
   α3 Na+/K+ ATPase (clone XVIF9-G10) | Mouse | 1:400 | Biomol, PA, USA. |
   NKCC1 | Rabbit | 1:1000 | Gift Dr RJ Turner, National Institute of Dental and Craniofacial Research, USA. |
   vGLUT1 (catalogue# 135 302) | Rabbit | 1:2000 | Synaptic Systems Goettingen, Germany. |
   vGLUT2 (catalogue# 135 402) | Rabbit | 1:2000 | Synaptic Systems Goettingen, Germany. |
Primary Antibodies – Neurotransmitters |  |  |  |
   CGRP (catalogue# RPN 1842) | Rabbit | 1:4000 | Amersham, UK. |
   Neuronal nitric oxide synthase (nNOS) | Sheep | 1:4000 | Gift Dr Colin Anderson, University of Melbourne, Australia. |
   Somatostatin (catalogue# AB5494) | Rabbit | 1:100 | Millipore, Australia. |
   Substance P (clone NC1) | Rat | 1:200 | Millipore, Australia. |
Primary Antibodies – Cytosolic Proteins |  |  |  |
   Calbindin D28k (number CB-38A) | Rabbit | 1:1000 | Swant Bellinzona, Switzerland. |
   Calretinin (number 7699/4) | Rabbit | 1:1000 | Swant Bellinzona, Switzerland. |
   Neurofilament 160KD (clone NN18) | Mouse | 1:400 | Millipore, Australia. |
   Parvalbumin (number 235) | Mouse | 1:400 | Swant Bellinzona, Switzerland. |
Secondary Antibodies (IgG, H+L, 2 mg/ml) | Â | Â | Â |
   AlexaFluor 488 or 594 anti-goat | Donkey | 1:200 | Molecular Probes Eugene, OR, USA |
   AlexaFluor 488 or 594 anti-mouse | Donkey | 1:200 | Molecular Probes Eugene, OR, US |
   AlexaFluor 488 or 594 anti-rabbit | Donkey | 1:200 | Molecular Probes Eugene, OR, USA |
   AlexaFluor 488 anti-rat | Goat | 1:200 | Molecular Probes Eugene, OR, USA |