Immunohistochemical characterization of nodose cough receptor neurons projecting to the trachea of guinea pigs

Cough

Table 2 Mean cell sizes of guinea pig nodose neurons.

Markers Commonly Expressed¹	Average Cell Perimeter (μm)		Markers Uncommonly Expressed²	Average Cell Perimeter (μm)
	Marker (+)	Marker(-)/FG(+)		Marker (+)	Marker(+)/FG(+)
NKCC1	113.9 ± 3.1*	None	Substance P	99.1 ± 3.7*	108.3 ± 5.9*
α1 Na⁺/K⁺ ATPase	109.3 ± 4.8*	131.3 ± 8.4	CGRP	90.0 ± 2.6*	92.9³ *
α3 Na⁺/K⁺ ATPase	139.1 ± 1.8	128.5 ± 5.1	Somatostatin	80.4 ± 2.2*	None
vGlut1	135.7 ± 2.6	126.8 ± 4.7	nNOS (IFCs)	98.3 ± 2.6*	109.5 ± 8.9*
vGlut2	109.6 ± 3.0*	138.6 ± 1.8	Calretinin	177.9 ± 2.6*	None
Neurofilament	142.1 ± 5.7	105.3 ± 2.4*	Calbindin	173.3 ± 2.1*	None
nNOS (all)	112.6 ± 2.9*	126.8 ± 5.9	Parvalbumin	None	None

¹ Defined as a marker that is expressed in more than 50 per cent of the FG traced neurons.
² Defined as a marker that is expressed in less than 50 per cent of the FG traced neurons.
³ Only one FG traced neuron expressed CGRP.
*P < 0.05, significantly different compared to the average size of FG traced neurons, Student's t-test (Note. The average somal perimeter of FG traced neurons = 137.3 ± 6.2).
Abbreviations: CGRP; Calcitonin Gene-Related Peptide; FG, Fluorogold; IFCs, Intensely Fluorescent Cells; nNOS, neuronal Nitric Oxide Synthase; vGlut, vesicular Glutamate transporter.